Journal: Epigenetics

Article Title: Nutrigenetics, epigenetics and gestational diabetes: consequences in mother and child

doi: 10.1080/15592294.2019.1582277

Figure Lengend Snippet: Studies investigating miRNAs in GDM and offspring.

Article Snippet: Houde 2014 [ ] , Cohort , 126 (27 GDM) , 2-h 75 g OGTT, GDM glucose ≥7.8 mmol/L according to WHO criteria , Placenta (foetal) , Bisulfite pyrosequencing qRT-PCR , Lower LPL DNA methylation levels in GDM. Negative correlation between LPL DNA methylation levels in GDM and maternal 2h glucose levels/HDL-C.

Techniques: Expressing, Clinical Proteomics, Next-Generation Sequencing, DNA Methylation Assay, Quantitative RT-PCR

Journal: Cancer Cell International

Article Title: Epidermal growth factor-mediated Rab25 pathway regulates integrin β1 trafficking in colon cancer

doi: 10.1186/s12935-018-0526-y

Figure Lengend Snippet: Integrin β1 and Rab25 expression in EGF-treated cells. a Integrin β1 and Rab25 expression was examined in HCT116 cells stimulated with 100 ng/mL EGF by western blotting. b , c Densitometric quantification of the data shown in a for b integrin β1 and c Rab25 ( CTL control)

Article Snippet: Rab25 FlexTube siRNA (Hs_RAB25_5, Qiagen, Hilden, Germany), RNAiMAX lipofectamine reagent (13778-030, Invitrogen), and Opti-MEM (Invitrogen) were mixed, incubated at room temperature for 20 min, and then added to cells.

Techniques: Expressing, Western Blot, Control

Journal: Cancer Cell International

Article Title: Epidermal growth factor-mediated Rab25 pathway regulates integrin β1 trafficking in colon cancer

doi: 10.1186/s12935-018-0526-y

Figure Lengend Snippet: Alterations in integrin β1 localization after Rab25 knockdown. a Integrin β1 and Rab25 expression was monitored by western blotting after fractionation. b Densitometric quantification of integrin β1 expression in mock and Rab25-knockdown cells. c Densitometric quantification of data shown in a ( CTL control)

Article Snippet: Rab25 FlexTube siRNA (Hs_RAB25_5, Qiagen, Hilden, Germany), RNAiMAX lipofectamine reagent (13778-030, Invitrogen), and Opti-MEM (Invitrogen) were mixed, incubated at room temperature for 20 min, and then added to cells.

Techniques: Knockdown, Expressing, Western Blot, Fractionation, Control

Journal: Cancer Cell International

Article Title: Epidermal growth factor-mediated Rab25 pathway regulates integrin β1 trafficking in colon cancer

doi: 10.1186/s12935-018-0526-y

Figure Lengend Snippet: Changes of integrin β1 expression in response to EGF stimulation and Rab25-knockdown. a , b Relative distribution of integrin β1 in a cytosol and b membrane following EGF stimulation in control ( CTL control, solid line) and Rab25-knockdown (siRab25, dotted line) cells

Article Snippet: Rab25 FlexTube siRNA (Hs_RAB25_5, Qiagen, Hilden, Germany), RNAiMAX lipofectamine reagent (13778-030, Invitrogen), and Opti-MEM (Invitrogen) were mixed, incubated at room temperature for 20 min, and then added to cells.

Techniques: Expressing, Knockdown, Membrane, Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: How to Translate DNA Methylation Biomarkers Into Clinical Practice

doi: 10.3389/fcell.2022.854797

Figure Lengend Snippet: Comparison of selected methods for DNAm analysis.

Article Snippet: Pyrosequencing , Sequencing of PCR amplicons of bisulfite-converted DNA , NA , + , ++ , - , + , — , - , ↔ , ↔.

Techniques: Comparison, Genome Wide, Methylation Sequencing, Next-Generation Sequencing, Microarray, Nanopore Sequencing, Sequencing, Methylation, Immunoprecipitation, Methylated DNA Immunoprecipitation, Binding Assay, Amplification, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction